Manipulation of CBTS1 Expression Alters Tobacco Resistance to Spodoptera frugiperda and Phytophthora nicotianae
نویسندگان
چکیده
Cembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role the biosynthesis of cembranoids directly determines cembranoid content tobacco. This study examined effect changing CBTS1 expression on pest Spodoptera frugiperda oomycete pathogen Phytophthora nicotianae. The CDS sequence was cloned into gene overexpression silencing vectors introduced (Nicotiana tabacum L. cv. TN90) obtain CBTS1-overexpression plants (CBTS1-OE) CBTS1-silenced (CBTS1-RI). Compared with control plants, cembratrien-ol (CBT-ol) increased 4.48 times CBTS1-OE but decreased 68% CBTS1-RI while that cembratrien-diol (CBT-diol) 3.17 76% plants. S. transgenic evaluated vitro toxicity test, results showed significantly improved reduced. P. nicotianae assessed detached leaf assay, enhanced, attenuated. Further analysis pathogen-related PR-1a, its expression. demonstrated manipulating could change alter their both
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ژورنال
عنوان ژورنال: Agronomy
سال: 2023
ISSN: ['2156-3276', '0065-4663']
DOI: https://doi.org/10.3390/agronomy13030845